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Objective:To evaluate the report quality and study characteristics of randomized controlled trials (RCT) published in the Chinese Journal of Health Management.Methods:All studies published in the Chinese Journal of Health Management from January 2007 to July 2019 were retrieved via Wanfang data. Two researchers screened the articles strictly according to the inclusion and exclusion criteria independently. Based on the Consolidated Standards of Reporting Trials (CONSORT) statement 2010, the report quality and characteristics of the articles (including the type of participants, recruiting site, interventions, outcome measure, etc.) were extracted and summarized. Then the report quality, study characteristics were analysed among three periods based on the publishing date (2008—2011 , 2012—2015 , 2016—2019). Results:Titles, abstracts, and full text manuscripts were screened against inclusion criteria by two independent reviewers and 57 studies were included. There were 13/25 items of the CONSORT statement were fulfilled completely, the other 8/25 items were partly fulfilled, and the else 4/25 items were not met the standards in these studies. There was statistically significant difference in the description ratio of ‘randomization sequence generation’ (16.7% vs. 47.6% vs. 66.7%), foundation (25.0% vs. 33.3% vs. 75.0%) and informed consent (50.0% vs. 76.2% vs. 100.0%) during the three periods (all P<0.05). There was no statistically significant difference in the proportion of chronic diseases (58.3% vs. 85.7% vs. 66.7%), the description ratio of participant flow (41.7% vs. 33.3% vs. 75.0%) and baseline data (83.3% vs. 95.2% vs. 100.0%) in the studies from different periods, however, the description situation of participant flow and baseline data has been improved along the time. Conclusion:According to the CONSORT statement, it is found that the quality of research in health management still needs to be improved, especially in the concealment of randomization and trial registration.
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Objective To search and analyze the DNA methylation-related genes of ?-thalassemia by oligonucleotide microarray in order to explore a new method for early diagnosis of ?-thalassemia.Methods The cord blood samples of 2 children with ?-thalassemia and 2 health children were detected by a chip containing human DNA methylation 30 178 oligonucleotide probes.The chip results were verified by the methods of methylation-specific PCR (MSP) and real-time PCR.The statistical significance of differentially expressed genes was found on non-supervised clustering (Hierarchical clustering).Results A total of 209 genetic differences (ratio≥2.0 or≤0.5) were showed by 2 groups of chips,and of them 113 genes were up-regulated and 96 genes were down-regulated.The results showed that the methylation-related gene erythroblastic leukemia viral (v-erb-a) was of hypermethylation compared with the normal blood.Conclusion A large number of differentially expressed genes are screened out by the technology of High-throughput DNA methylation of the gene chip in thalassemia.The DNA methylation-related gene of v-erb-a is of hypermethylation in thalassemia.Our methods offers a new idea and approach for prenatal diagnosis for thalassemia by the DNA methylation-related microarray.